Red Cycler 5C Bedienungsanleitung

The World’s fastest Real-Time PCR Thermal Cycler User Manual This Manual Copyright © 2014 BJS Biotechnologies

P a g e | 10 XXPRESS USER MANUAL - 1.1 PCR Performance Thermal Ramp Rate Thermal Uniformity Fluorescence Detection Maximum Ra

P a g e | 11 XXPRESS USER MANUAL - 1.1 5 Safety Precautions and Limitations of Use It is essential that all users of this equipment hav

P a g e | 12 XXPRESS USER MANUAL - 1.1 Unit Installation and Operating Environment DANGER WARNING The xxpress unit is designed for indoor labor

P a g e | 13 XXPRESS USER MANUAL - 1.1 General Operating Precautions DANGER Ensure that the power is switched off at both the AC mains supply

P a g e | 14 XXPRESS USER MANUAL - 1.1 Unit Maintenance and Serviceability DANGER WARNING There are no user or operator serviceable parts insid

P a g e | 15 XXPRESS USER MANUAL - 1.1 6 Unit Installation Before installing the xxpress unit, please check that the delivery is compl

P a g e | 16 XXPRESS USER MANUAL - 1.1 Install and test the xxpress unit using the following procedure: 1) Place the unit on the suitably selec

P a g e | 17 XXPRESS USER MANUAL - 1.1 7 Unit Operation WARNING Please ensure that you have read and fully understood all of the Safety Precaut

P a g e | 18 XXPRESS USER MANUAL - 1.1 • The unit is now powered and so the rear fan may operate depending on the temperature of the

P a g e | 19 XXPRESS USER MANUAL - 1.1 Figure 2 Close the screen by touching the  symbol in the top right corner of the screen. The next

P a g e | 2 XXPRESS USER MANUAL - 1.1 xxpress, xxplates, xxsealer, and their respective logos are registered trademarks of BJS Biotechnologies

P a g e | 20 XXPRESS USER MANUAL - 1.1 thermal cycling profile shown in later screens to match the experimental need). Figure 3 • I

P a g e | 21 XXPRESS USER MANUAL - 1.1 • The first selection is to choose between a PROBE based test or one the uses SYBR GREEN based signal

P a g e | 22 XXPRESS USER MANUAL - 1.1 • Once the chosen supplier and type of chemistry is selected (Figure 4b) then the user proceeds to

P a g e | 23 XXPRESS USER MANUAL - 1.1 • Looking at our example 3-Step PCR, we chose a SYBR Green based test and Kapa SYBR 2G Fast Hot

P a g e | 24 XXPRESS USER MANUAL - 1.1 • In the third step the temperature is increased to 72°C, the ramp rate is again 10°C per

P a g e | 25 XXPRESS USER MANUAL - 1.1 Figure 5b • Touch the tick or  cross to accept or reject the changes as before. • At this sta

P a g e | 26 XXPRESS USER MANUAL - 1.1 Figure 5d • Touch the MELT tile to add a Melt Curve cycle to the experiment, see Figure 5d. • The M

P a g e | 27 XXPRESS USER MANUAL - 1.1 Figure 6 • Chose the desired test plate size by touching it. xxpress has a range of three

P a g e | 28 XXPRESS USER MANUAL - 1.1 • These wells can be labelled with information to help the analysis of the results. To select a grou

P a g e | 29 XXPRESS USER MANUAL - 1.1  REFERENCE GENE a sample of known response for comparison.  STANDARD CURVE where that process is

P a g e | 3 XXPRESS USER MANUAL - 1.1 Contents 1 Glossary of Terms and Abbreviations ...

P a g e | 30 XXPRESS USER MANUAL - 1.1 • Touch the tick will accept the information and then the process can be repeated as necessary to labe

P a g e | 31 XXPRESS USER MANUAL - 1.1 • Once all the information is entered as shown in Figure 7f, touch again the forward arrow at

P a g e | 32 XXPRESS USER MANUAL - 1.1 Figure 9 • Once the experiment is initiated xxpress performs a number of checks on the plate

P a g e | 33 XXPRESS USER MANUAL - 1.1 Figure 9a 7.2.3 Designing a New Melt-Curve Experiment • Creating a Melt-Curve Experiment is

P a g e | 34 XXPRESS USER MANUAL - 1.1 Figure 11 • The Chemistry screen is simpler as just the signalling type needs to be defined to allow

P a g e | 35 XXPRESS USER MANUAL - 1.1 Figure 12a • The example in Figure 12 above shows a Melt that will start at 40°C after a

P a g e | 36 XXPRESS USER MANUAL - 1.1 Figure 14 • The plate layout can be created as shown earlier or it can be recalled from th

P a g e | 37 XXPRESS USER MANUAL - 1.1 Figure 14b • Figure 14b shows the confirmation screen that gives the user the opportunity to review

P a g e | 38 XXPRESS USER MANUAL - 1.1 7.2.4 Running the Experiment • Before running an experiment the sample xxplate needs to be pre

P a g e | 39 XXPRESS USER MANUAL - 1.1 • Place the xxplate onto the slides ensuring that it correctly located so that the slides engage into al

P a g e | 4 XXPRESS USER MANUAL - 1.1 1 Glossary of Terms and Abbreviations ANSI BJS Bio American National Standards Institute BJS Biotechnolo

P a g e | 40 XXPRESS USER MANUAL - 1.1 • During the operation it is normal for the unit to issue a short hum from the cooling compressors every

P a g e | 41 XXPRESS USER MANUAL - 1.1 • There is a manual adjustment that can be used to adjust the Ct threshold value by eye.

P a g e | 42 XXPRESS USER MANUAL - 1.1 • Touching the DIFFERENTIATE tile on the right will give a display like the one shown in Figure 17b. Fig

P a g e | 43 XXPRESS USER MANUAL - 1.1 8 Maintenance and Servicing Although the xxpress unit does not require any scheduled maintenance or servi

P a g e | 44 XXPRESS USER MANUAL - 1.1 Figure 18 1. Remove the AC plug from the power inlet module 2. Use a small flat bladed screwdriver to

P a g e | 45 XXPRESS USER MANUAL - 1.1 8.3 Decontamination Procedure The unit and accessories should be decontaminated using the following proce

P a g e | 46 XXPRESS USER MANUAL - 1.1 Certificate of Decontamination We respect the health and safety of our clients and employees,

P a g e | 47 XXPRESS USER MANUAL - 1.1 9 Warranty and Returns BJS Biotechnologies Ltd. warrants the xxpress product, when purchased new

P a g e | 48 XXPRESS USER MANUAL - 1.1 10 Product Disposal At end-of-life, this product must be disposed of in accordance with your local authori

P a g e | 5 XXPRESS USER MANUAL - 1.1 2 Symbols Used in this Instruction Manual The following advisory symbols are used in this manual. Tabl

P a g e | 6 XXPRESS USER MANUAL - 1.1 3 Unit Description The xxpress Real-Time Thermal cycler provides a safe and controlled method heati

P a g e | 7 XXPRESS USER MANUAL - 1.1 Table 2: Unit Features 1 xxpress Power On/Off Button 2 Front Door 3 Front Door Release

P a g e | 8 XXPRESS USER MANUAL - 1.1 The xxpress unit is designed to be used with a range of sample plates and accessories – some of which are l

P a g e | 9 XXPRESS USER MANUAL - 1.1 4 Technical Specification Model Type Model Name Model No xxpress XP-1001-01-1 Physical Unit Pr